Morphological and DNA Polymorphism Analyses of Fusarium solani Isolated from Gyrinops versteegii in the West Nusa Tenggara Forest

Wangiyana, I Gde Adi Suryawan and Nugraheni, Yosephin Martha Maria Anita and Anggadhania, Lutfi and Soetarto, Endang Sutariningsih (2021) Morphological and DNA Polymorphism Analyses of Fusarium solani Isolated from Gyrinops versteegii in the West Nusa Tenggara Forest. [Artefact]

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Abstract

Fusarium is the most common bio-induction agent in agarwood cultivation. Taxonomic study of Fusarium could reveal characteristics of Fusarium species that have high compatibility with agarwood, such as Gyrinops versteegii. DNA fingerprinting can be used in addition to the morphological data in a polyphasic approach for understanding the taxonomy of Fusarium. Thus, this study aims to infer the genetic diversity of selected Fusarium solani isolated from G. versteegii collected at four localities in West Nusa Tenggara Province using a polyphasic approach by comparing the dendrograms generated from morphological data and RAPD profiles. Four F. solani isolates (LU, LT, LB and BM) representing each of the collection locality were subjected to morphological observations and RAPD-PCR profiling. The fungal isolates were grown on three different media for morphological observations on the characteristics of colony, hyphae and conidia. All isolates produced colony with the largest diameter and highest conidia concentration on the bean sprout extract medium. Based on the RAPD profiles, isolate BM showed the highest number of polymorphic fragments among all isolates. Dendrograms generated from both morphological and molecular data were similar in showing that isolate BM was more dissimilar to other isolates. However, the dendrograms were slightly different in which isolate LT was more similar to isolate LB in the RAPD-based dendrogram, while it was more similar to isolate LU in the dendrogram generated from morphological characters. We concluded that molecular characterisation with RAPD analysis supports morphological characterisation of F. solani isolates in a polyphasic taxonomy.

Item Type:	Artefact
Uncontrolled Keywords:	DNA polymorphism; Fusarium solani; Gyrinops versteegii; morphological character
Subjects:	Q Science > Q Science (General)
Divisions:	Biro > Biro Administrasi Umum
Depositing User:	Admin Jafung UNDIKMA
Date Deposited:	12 May 2023 07:33
Last Modified:	12 May 2023 07:33
URI:	http://repository.undikma.ac.id/id/eprint/951

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